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Gene : |
MS4A1 |
Gene ID : |
931 |
Uniprot ID : |
P11836 |
Alternative Name : |
MS4A1, CD20 |
Format : |
Purified |
Amount : |
100 µg |
Clone name : |
L26 + IGEL/773 |
Isotype : |
Mouse IgG2a, kappa + Mouse IgG2a, kappa |
Immunogen Information : |
Human tonsil B cells (L26); Recombinant human MS4A1 protein (IGEL/773) |
Recognizes a protein of 30-33kDa, which is identified as CD20. Its epitope is located in the cytoplasmic domain of CD20 and was, therefore, ascribed as CD20cy in the 5th Workshop. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin's disease, particularly of lymphocyte predominant type.
Flow Cytometry (5-10ul/million cells); ,Immunofluorescence (1:50-1:100); ,Immunohistochemistry (Formalin-fixed) (1:100-1:200 for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);